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Every time you puncture the stopper with a needle, you introduce bacteria

The interplay between genetics and environmental factors plays a crucial role in the development and progression of the disease

Some metabolic enzymes, such as succinate dehydrogenase (SDH), fumarate hydratase (FH), IDH and pyruvate kinase 2 (PKM2) are likely to activate HIF-1 pathway by stabilizing HIF-1, therefore enhancing cancer metastasis (258)

According to the results of the Rosetta Cartesian_ddG and Flex_ddG, we manually checked the PV2 and PV3 variants predicted to be highly stable, exhibit low binding free energy and are located outside the CDR regions, and those having pAcF facing outward were selected for experimental characterization, including PV2 variants S8pAcF, S26pAcF, S71pAcF, S74pAcF, Q82pAcF, S85pAcF and PV3 variants S8pAcF, S26pAcF, S71pAcF, N77pAcF, S85pAcF, K87pAcF (Fig